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DuPont de Nemours
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Image Search Results
Journal: International journal of oncology
Article Title: Carnosol induces apoptosis through generation of ROS and inactivation of STAT3 signaling in human colon cancer HCT116 cells.
doi: 10.3892/ijo.2014.2281
Figure Lengend Snippet: Figure 2. Effect of carnosol on cellular markers of apoptosis in HCT116 cells. Cells were treated with indicated concentrations of carnosol for 24 h. (A) Immunoblot analysis for detecting the levels of cleaved caspase-9, and -3, and cleaved PARP. (B) After 24 h of incubation with carnosol (indicated concentrations) or etoposide (85 µM), the caspase-3 activity assay was performed as described in Materials and methods. (C) Western blot analysis of p53 and Mdm2 expression. (D) The expression level of Bcl-2, Bcl-xl and Bax. Data are representative of three different experiments.
Article Snippet: Primary antibody against each of p53, murine double minute-2 (
Techniques: Western Blot, Incubation, Caspase-3 Activity Assay, Expressing